Fluorescent labeling of tRNAs for dynamics experiments

Transfer RNAs (tRNAs) are substrates for complex enzymes, such as aminoacyl-tRNA synthetases and ribosomes, and play an essential role in translation of genetic information into protein sequences. Here we describe a general method for labeling tRNAs with fluorescent dyes, so that the activities and...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Betteridge, Thu, Liu, Hanqing, Gamper, Howard, Kirillov, Stanislav, Cooperman, Barry S., Hou, Ya-Ming
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Cold Spring Harbor Laboratory Press 2007
Pynciau:
Method
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1950756/
https://ncbi.nlm.nih.gov/pubmed/17652134
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.475407
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