Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI

BsrDI and BtsI restriction endonucleases recognize and cleave double-strand DNA at the sequences GCAATG (2/0) and GCAGTG (2/0), respectively. We have purified and partially characterized these two enzymes, and analyzed the genes that encode them. BsrDI and BtsI are unusual in two respects: each clea...

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Bibliografiska uppgifter
Huvudupphovsmän: Xu, Shuang-Yong, Zhu, Zhenyu, Zhang, Penghua, Chan, Siu-Hong, Samuelson, James C., Xiao, Jianping, Ingalls, Debra, Wilson, Geoffrey G.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2007
Ämnen:
Nucleic Acid Enzymes
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1950550/
https://ncbi.nlm.nih.gov/pubmed/17586812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm481
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