Loading...

The isolation of strand-specific nicking endonucleases from a randomized SapI expression library

The Type IIS restriction endonuclease SapI recognizes the DNA sequence 5′-GCTCTTC-3′ (top strand by convention) and cleaves downstream (N1/N4) indicating top- and bottom-strand spacing, respectively. The asymmetric nature of DNA recognition presented the possibility that one, if not two, nicking var...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Main Authors: Samuelson, James C., Zhu, Zhenyu, Xu, Shuang-yong
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2004
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC484165/
https://ncbi.nlm.nih.gov/pubmed/15247348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh674
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!