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Natural and engineered nicking endonucleases—from cleavage mechanism to engineering of strand-specificity
Restriction endonucleases (REases) are highly specific DNA scissors that have facilitated the development of modern molecular biology. Intensive studies of double strand (ds) cleavage activity of Type IIP REases, which recognize 4–8 bp palindromic sequences, have revealed a variety of mechanisms of...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3017599/ https://ncbi.nlm.nih.gov/pubmed/20805246 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq742 |
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