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Natural and engineered nicking endonucleases—from cleavage mechanism to engineering of strand-specificity

Restriction endonucleases (REases) are highly specific DNA scissors that have facilitated the development of modern molecular biology. Intensive studies of double strand (ds) cleavage activity of Type IIP REases, which recognize 4–8 bp palindromic sequences, have revealed a variety of mechanisms of...

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Detalles Bibliográficos
Main Authors: Chan, Siu-Hong, Stoddard, Barry L., Xu, Shuang-yong
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3017599/
https://ncbi.nlm.nih.gov/pubmed/20805246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq742
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