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The isolation of strand-specific nicking endonucleases from a randomized SapI expression library
The Type IIS restriction endonuclease SapI recognizes the DNA sequence 5′-GCTCTTC-3′ (top strand by convention) and cleaves downstream (N1/N4) indicating top- and bottom-strand spacing, respectively. The asymmetric nature of DNA recognition presented the possibility that one, if not two, nicking var...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2004
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC484165/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15247348/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkh674 |
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