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Dissecting the multistep reaction pathway of an RNA enzyme by single-molecule kinetic “fingerprinting”

Single-molecule FRET is a powerful tool for probing the kinetic mechanism of a complex enzymatic reaction. However, not every reaction intermediate can be identified via a distinct FRET value, making it difficult to fully dissect a multistep reaction pathway. Here, we demonstrate a method using sequ...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Liu, Shixin, Bokinsky, Gregory, Walter, Nils G., Zhuang, Xiaowei
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2007
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1937518/
https://ncbi.nlm.nih.gov/pubmed/17496145
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0610597104
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