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Dissecting the multistep reaction pathway of an RNA enzyme by single-molecule kinetic “fingerprinting”
Single-molecule FRET is a powerful tool for probing the kinetic mechanism of a complex enzymatic reaction. However, not every reaction intermediate can be identified via a distinct FRET value, making it difficult to fully dissect a multistep reaction pathway. Here, we demonstrate a method using sequ...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
2007
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1937518/ https://ncbi.nlm.nih.gov/pubmed/17496145 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0610597104 |
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