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Nucleoside alpha-thiotriphosphates, polymerases and the exonuclease III analysis of oligonucleotides containing phosphorothioate linkages

The use of DNA polymerases to incorporate phosphorothioate linkages into DNA, and the use of exonuclease III to determine where those linkages have been incorporated, are re-examined in this work. The results presented here show that exonuclease III degrades single-stranded DNA as a substrate and di...

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Autori principali: Yang, Zunyi, Sismour, A. Michael, Benner, Steven A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1888802/
https://ncbi.nlm.nih.gov/pubmed/17452363
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm168
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