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Nucleoside alpha-thiotriphosphates, polymerases and the exonuclease III analysis of oligonucleotides containing phosphorothioate linkages

The use of DNA polymerases to incorporate phosphorothioate linkages into DNA, and the use of exonuclease III to determine where those linkages have been incorporated, are re-examined in this work. The results presented here show that exonuclease III degrades single-stranded DNA as a substrate and di...

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Detalhes bibliográficos
Main Authors: Yang, Zunyi, Sismour, A. Michael, Benner, Steven A.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1888802/
https://ncbi.nlm.nih.gov/pubmed/17452363
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm168
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