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A new strategy for assessing selenoprotein function: siRNA knockdown/knock-in targeting the 3′-UTR

Selenocysteine insertion into protein in mammalian cells requires RNA elements in the 3′-untranslated regions (3′-UTRs) of selenoprotein genes. The occurrence of these conserved sequences should make selenoproteins particularly amenable for knockdown/knock-in strategies to examine selenoprotein func...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Yoo, Min-Hyuk, Xu, Xue-Ming, Turanov, Anton A., Carlson, Bradley A., Gladyshev, Vadim N., Hatfield, Dolph L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2007
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1869036/
https://ncbi.nlm.nih.gov/pubmed/17468436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.533007
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