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The 30-kDa C-terminal domain of the RecB protein is critical for the nuclease activity, but not the helicase activity, of the RecBCD enzyme from Escherichia coli
The RecBCD enzyme from Escherichia coli is an ATP-dependent helicase and an ATP-stimulated nuclease. The 3′ → 5′ exonuclease activity on double-stranded DNA is suppressed when the enzyme encounters a recombinational hot spot, called chi (χ). We have prepared a RecB deletion mutant (RecB(1–929)) by u...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The National Academy of Sciences
1998
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC18645/ https://ncbi.nlm.nih.gov/pubmed/9448271 |
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