Cloning and nucleotide sequence of the Streptococcus pneumoniae hyaluronidase gene and purification of the enzyme from recombinant Escherichia coli.

A gene bank of Sau3A1-generated Streptococcus pneumoniae type 23 DNA fragments was constructed in Escherichia coli K-12 with the low-copy-number cosmid vector pOU61cos. Clone lysates were screened by immunoblotting using a mouse antiserum raised against a crude pneumococcal hyaluronidase preparation...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Berry, A M, Lock, R A, Thomas, S M, Rajan, D P, Hansman, D, Paton, J C
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1994
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC186229/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8112843/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.62.3.1101-1108.1994
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