Cloning and expression of the Vibrio cholerae neuraminidase gene nanH in Escherichia coli.

A cosmid gene bank of Vibrio cholerae 395, classical Ogawa, was screened in Escherichia coli HB101 for expression of the vibrio neuraminidase (NANase) gene nanH (N-acylneuraminate glycohydrolase). Positive clones were identified by their ability to cleave the fluorogenic NANase substrate 2'-(4-...

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Main Authors: Vimr, E R, Lawrisuk, L, Galen, J, Kaper, J B
Format: Artigo
Sprog:Inglês
Udgivet: 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC210993/
https://ncbi.nlm.nih.gov/pubmed/2832365
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