Cloning and expression of the Vibrio cholerae neuraminidase gene nanH in Escherichia coli.

A cosmid gene bank of Vibrio cholerae 395, classical Ogawa, was screened in Escherichia coli HB101 for expression of the vibrio neuraminidase (NANase) gene nanH (N-acylneuraminate glycohydrolase). Positive clones were identified by their ability to cleave the fluorogenic NANase substrate 2'-(4-methy...

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Dades bibliogràfiques

Publicat a:	J Bacteriol
Autors principals:	Vimr, E R, Lawrisuk, L, Galen, J, Kaper, J B
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1988
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210993/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2832365/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.4.1495-1504.1988
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