Cloning and expression of the Vibrio cholerae neuraminidase gene nanH in Escherichia coli.
A cosmid gene bank of Vibrio cholerae 395, classical Ogawa, was screened in Escherichia coli HB101 for expression of the vibrio neuraminidase (NANase) gene nanH (N-acylneuraminate glycohydrolase). Positive clones were identified by their ability to cleave the fluorogenic NANase substrate 2'-(4-methy...
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| Publicado en: | J Bacteriol |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1988
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210993/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2832365/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.4.1495-1504.1988 |
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