Cloning and nucleotide sequence of the Streptococcus pneumoniae hyaluronidase gene and purification of the enzyme from recombinant Escherichia coli.
A gene bank of Sau3A1-generated Streptococcus pneumoniae type 23 DNA fragments was constructed in Escherichia coli K-12 with the low-copy-number cosmid vector pOU61cos. Clone lysates were screened by immunoblotting using a mouse antiserum raised against a crude pneumococcal hyaluronidase preparation...
Sparad:
| I publikationen: | Infect Immun |
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| Huvudupphov: | , , , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
American Society for Microbiology (ASM)
1994
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC186229/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8112843/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.62.3.1101-1108.1994 |
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