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Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA

The extrinsic fluorescence dye 8-anilino-1-naphthalene sulfonate (ANS) is widely used for probing conformational changes in proteins, yet no detailed structure of ANS bound to any protein has been reported so far. ANS has been successfully used to monitor the induced-fit mechanism of MurA [UDPGlcNAc...

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Detalhes bibliográficos
Main Authors: Schönbrunn, Ernst, Eschenburg, Susanne, Luger, Karolin, Kabsch, Wolfgang, Amrhein, Nikolaus
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2000
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC18605/
https://ncbi.nlm.nih.gov/pubmed/10823915
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