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Force Unfolding Kinetics of RNA Using Optical Tweezers. I. Effects of Experimental Variables on Measured Results

Experimental variables of optical tweezers instrumentation that affect RNA folding/unfolding kinetics were investigated. A model RNA hairpin, P5ab, was attached to two micron-sized beads through hybrid RNA/DNA handles; one bead was trapped by dual-beam lasers and the other was held by a micropipette...

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Detaylı Bibliyografya
Asıl Yazarlar: Wen, Jin-Der, Manosas, Maria, Li, Pan T. X., Smith, Steven B., Bustamante, Carlos, Ritort, Felix, Tinoco, Ignacio
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Biophysical Society 2007
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1852336/
https://ncbi.nlm.nih.gov/pubmed/17293410
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.094052
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