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Local RNA structural changes induced by crystallization are revealed by SHAPE
We present a simple approach to locate sites that undergo conformational changes upon crystallization by comparative structural mapping of the same RNA in three different environments. As a proof of principle, we probed the readily crystallized P4–P6ΔC209 domain from the Tetrahymena thermophila grou...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Cold Spring Harbor Laboratory Press
2007
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1831858/ https://ncbi.nlm.nih.gov/pubmed/17299128 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.400207 |
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