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Rapid Method for Direct Extraction of mRNA from Seeded Soils

A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by vigorously shaking the washed soil in a 4 M guanidine thiocyanate solutio...

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Bibliografski detalji
Glavni autori: Tsai, Yu-Li, Park, Marie J., Olson, Betty H.
Format: Artigo
Jezik:Inglês
Izdano: 1991
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC182792/
https://ncbi.nlm.nih.gov/pubmed/16348441
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