Rapid method for direct extraction of DNA from soil and sediments.

A rapid method for the direct extraction of DNA from soil and sediments was developed. The indigenous microorganisms in the soil and sediments were lysed by using lysozyme and a freeze-thaw procedure. The lysate was extracted with sodium dodecyl sulfate and phenol-chloroform. In addition to a high r...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Tsai, Y L, Olson, B H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182847/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1647749/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.4.1070-1074.1991
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