Rapid method for direct extraction of DNA from soil and sediments.
A rapid method for the direct extraction of DNA from soil and sediments was developed. The indigenous microorganisms in the soil and sediments were lysed by using lysozyme and a freeze-thaw procedure. The lysate was extracted with sodium dodecyl sulfate and phenol-chloroform. In addition to a high r...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1991
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182847/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1647749/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.4.1070-1074.1991 |
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