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Rapid Method for Direct Extraction of mRNA from Seeded Soils

A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by vigorously shaking the washed soil in a 4 M guanidine thiocyanate solutio...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Tsai, Yu-Li, Park, Marie J., Olson, Betty H.
פורמט: Artigo
שפה:Inglês
יצא לאור: 1991
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC182792/
https://ncbi.nlm.nih.gov/pubmed/16348441
תגים: הוספת תג
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