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Analysis of Dictyostelium mucoroides Macrocysts by Using a Simple Breakage and Fractionation Procedure

A method is described for the breakage and fractionation of Dictyostelium macrocysts. It involves vortexing with large glass beads to disrupt the macrocyst wall followed by differential low-speed centrifugation. It yields fractions containing purified wall material and intact endocytes. The endocyte...

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Hlavní autoři: North, Michael J., Cotter, David A.
Médium: Artigo
Jazyk:Inglês
Vydáno: 1991
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC182703/
https://ncbi.nlm.nih.gov/pubmed/16348399
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