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Analysis of Dictyostelium mucoroides Macrocysts by Using a Simple Breakage and Fractionation Procedure
A method is described for the breakage and fractionation of Dictyostelium macrocysts. It involves vortexing with large glass beads to disrupt the macrocyst wall followed by differential low-speed centrifugation. It yields fractions containing purified wall material and intact endocytes. The endocyte...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1991
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC182703/ https://ncbi.nlm.nih.gov/pubmed/16348399 |
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