Analysis of Dictyostelium mucoroides Macrocysts by Using a Simple Breakage and Fractionation Procedure
A method is described for the breakage and fractionation of Dictyostelium macrocysts. It involves vortexing with large glass beads to disrupt the macrocyst wall followed by differential low-speed centrifugation. It yields fractions containing purified wall material and intact endocytes. The endocyte...
Saved in:
| Published in: | Appl Environ Microbiol |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1991
|
| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182703/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348399/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.1.307-310.1991 |
| Tags: |
No Tags, Be the first to tag this record!
|