Analysis of Dictyostelium mucoroides Macrocysts by Using a Simple Breakage and Fractionation Procedure
A method is described for the breakage and fractionation of Dictyostelium macrocysts. It involves vortexing with large glass beads to disrupt the macrocyst wall followed by differential low-speed centrifugation. It yields fractions containing purified wall material and intact endocytes. The endocyte...
Gardado en:
| Publicado en: | Appl Environ Microbiol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1991
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182703/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348399/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.1.307-310.1991 |
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