A simple RNA probe system for analysis of Listeria monocytogenes polymerase chain reaction products.

The synthesis of an RNA probe specific for the hlyA gene of Listeria monocytogenes by in vitro transcription from a polymerase chain reaction (PCR)-generated template incorporating bacteriophage T7 promoter sequences is described. This simple method produced a high yield of RNA which hybridized spec...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Blais, B W, Phillippe, L M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182368/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8215354/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.9.2795-2800.1993
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