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A simple RNA probe system for analysis of Listeria monocytogenes polymerase chain reaction products.

The synthesis of an RNA probe specific for the hlyA gene of Listeria monocytogenes by in vitro transcription from a polymerase chain reaction (PCR)-generated template incorporating bacteriophage T7 promoter sequences is described. This simple method produced a high yield of RNA which hybridized spec...

詳細記述

保存先:
書誌詳細
出版年:Appl Environ Microbiol
主要な著者: Blais, B W, Phillippe, L M
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology (ASM) 1993
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182368/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8215354/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.9.2795-2800.1993
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