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A simple RNA probe system for analysis of Listeria monocytogenes polymerase chain reaction products.

The synthesis of an RNA probe specific for the hlyA gene of Listeria monocytogenes by in vitro transcription from a polymerase chain reaction (PCR)-generated template incorporating bacteriophage T7 promoter sequences is described. This simple method produced a high yield of RNA which hybridized spec...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Appl Environ Microbiol
Egile Nagusiak: Blais, B W, Phillippe, L M
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology (ASM) 1993
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182368/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8215354/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.9.2795-2800.1993
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