Using antibody catalysis to study the outcome of multiple evolutionary trials of a chemical task

Catalytic aldolase antibodies generated by immunization with two different, but structurally related, β-diketone haptens were cloned and sequenced to study similarities and differences between independently evolved catalysts. Kinetic and sequence analysis coupled with mutagenesis, structural, and mo...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Karlstrom, Amelie, Zhong, Guofu, Rader, Christoph, Larsen, Nicholas A., Heine, Andreas, Fuller, Roberta, List, Benjamin, Tanaka, Fujie, Wilson, Ian A., Barbas, Carlos F., Lerner, Richard A.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2000
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC18110/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10760259/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.8.3878
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