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Using antibody catalysis to study the outcome of multiple evolutionary trials of a chemical task

Catalytic aldolase antibodies generated by immunization with two different, but structurally related, β-diketone haptens were cloned and sequenced to study similarities and differences between independently evolved catalysts. Kinetic and sequence analysis coupled with mutagenesis, structural, and mo...

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Autors principals: Karlstrom, Amelie, Zhong, Guofu, Rader, Christoph, Larsen, Nicholas A., Heine, Andreas, Fuller, Roberta, List, Benjamin, Tanaka, Fujie, Wilson, Ian A., Barbas, Carlos F., Lerner, Richard A.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 2000
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC18110/
https://ncbi.nlm.nih.gov/pubmed/10760259
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