Rapid conditional knock-down–knock-in system for mammalian cells

RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound phenotypes, as many proteins combine multiple functions within one molecule and coordinate the assembly...

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Hlavní autoři: Hölzel, Michael, Rohrmoser, Michaela, Orban, Mathias, Hömig, Cornelia, Harasim, Thomas, Malamoussi, Anastassia, Gruber-Eber, Anita, Heissmeyer, Vigo, Bornkamm, Georg, Eick, Dirk
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2007
Témata:
Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1807947/
https://ncbi.nlm.nih.gov/pubmed/17169998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl1055
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