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Atomic force microscopy captures length phenotypes in single proteins

We use single-protein atomic force microscopy techniques to detect length phenotypes in an Ig module. To gain amino acid resolution, we amplify the mechanical features of a single module by engineering polyproteins composed of up to 12 identical repeats. We show that on mechanical unfolding, mutant...

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Autors principals: Carrion-Vazquez, Mariano, Marszalek, Piotr E., Oberhauser, Andres F., Fernandez, Julio M.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC18026/
https://ncbi.nlm.nih.gov/pubmed/10500169
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