載入...

Combining localized PCR mutagenesis and natural transformation in direct genetic analysis of a transcriptional regulator gene, pobR.

We present a procedure for efficient random mutagenesis of selected genes in a bacterial chromosome. The method combines PCR replication errors with the uptake of PCR-amplified DNA via natural transformation. Cloning of PCR fragments is not required, since mutations are transferred directly to the c...

全面介紹

Na minha lista:
書目詳細資料
Main Authors: Kok, R G, D'Argenio, D A, Ornston, L N
格式: Artigo
語言:Inglês
出版: 1997
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC179249/
https://ncbi.nlm.nih.gov/pubmed/9209043
標簽: 添加標簽
沒有標簽, 成為第一個標記此記錄!