Combining localized PCR mutagenesis and natural transformation in direct genetic analysis of a transcriptional regulator gene, pobR.

We present a procedure for efficient random mutagenesis of selected genes in a bacterial chromosome. The method combines PCR replication errors with the uptake of PCR-amplified DNA via natural transformation. Cloning of PCR fragments is not required, since mutations are transferred directly to the c...

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Publicat a:	J Bacteriol
Autors principals:	Kok, R G, D'Argenio, D A, Ornston, L N
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1997
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC179249/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9209043/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.13.4270-4276.1997
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