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Combining localized PCR mutagenesis and natural transformation in direct genetic analysis of a transcriptional regulator gene, pobR.

We present a procedure for efficient random mutagenesis of selected genes in a bacterial chromosome. The method combines PCR replication errors with the uptake of PCR-amplified DNA via natural transformation. Cloning of PCR fragments is not required, since mutations are transferred directly to the c...

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Bibliografiska uppgifter
Huvudupphovsmän: Kok, R G, D'Argenio, D A, Ornston, L N
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1997
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC179249/
https://ncbi.nlm.nih.gov/pubmed/9209043
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