Ca(2+) Selectivity of a Chemically Modified OmpF with Reduced Pore Volume

We studied an E. coli OmpF mutant (LECE) containing both an EEEE-like locus, typical of Ca(2+) channels, and an accessible and reactive cysteine. After chemical modification with the cysteine-specific, negatively charged (−1e) reagents MTSES or glutathione, this LECE mutant was tested for Ca(2+) ver...

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Main Authors: Miedema, Henk, Vrouenraets, Maarten, Wierenga, Jenny, Gillespie, Dirk, Eisenberg, Bob, Meijberg, Wim, Nonner, Wolfgang
Formato: Artigo
Idioma:Inglês
Publicado: Biophysical Society 2006
Assuntos:
Channels, Receptors, and Electrical Signaling
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1779923/
https://ncbi.nlm.nih.gov/pubmed/16997866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.087114
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