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Structural basis of the conversion of T4 lysozyme into a transglycosidase by reengineering the active site
In contrast to hen egg-white lysozyme, which retains the β-configuration of the substrate in the product, T4 lysozyme (T4L) is an inverting glycosidase. The substitution Thr-26 → His, however, converts T4L from an inverting to a retaining enzyme. It is shown here that the Thr-26 → His mutant is also...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
The National Academy of Sciences
1999
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC17713/ https://ncbi.nlm.nih.gov/pubmed/10430876 |
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