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Structural basis of the conversion of T4 lysozyme into a transglycosidase by reengineering the active site

In contrast to hen egg-white lysozyme, which retains the β-configuration of the substrate in the product, T4 lysozyme (T4L) is an inverting glycosidase. The substitution Thr-26 → His, however, converts T4L from an inverting to a retaining enzyme. It is shown here that the Thr-26 → His mutant is also...

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Hlavní autoři: Kuroki, Ryota, Weaver, Larry H., Matthews, Brian W.
Médium: Artigo
Jazyk:Inglês
Vydáno: The National Academy of Sciences 1999
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC17713/
https://ncbi.nlm.nih.gov/pubmed/10430876
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