Introduction of unmarked mutations in the Helicobacter pylori vacA gene with a sucrose sensitivity marker.
Research on Helicobacter pylori has been hindered by the lack of useful genetic tools. Using the sacB gene of Bacillus subtilis, we developed a sucrose-based counterselection system that allows introduction of unmarked mutations in H. pylori. A kan-sacB cassette, consisting of the sacB gene expresse...
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| Pubblicato in: | Infect Immun |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
1997
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC175249/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9125586/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.65.5.1949-1952.1997 |
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