Introduction of unmarked mutations in the Helicobacter pylori vacA gene with a sucrose sensitivity marker.

Research on Helicobacter pylori has been hindered by the lack of useful genetic tools. Using the sacB gene of Bacillus subtilis, we developed a sucrose-based counterselection system that allows introduction of unmarked mutations in H. pylori. A kan-sacB cassette, consisting of the sacB gene expresse...

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Detalles Bibliográficos

Publicado en:	Infect Immun
Autores principales:	Copass, M, Grandi, G, Rappuoli, R
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1997
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC175249/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9125586/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.65.5.1949-1952.1997
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