Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase

Insertion of the T3 DNA polymerase thioredoxin binding domain (TBD) into the distantly related thermostable Taq DNA polymerase at an analogous position in the thumb domain, converts the Taq DNA polymerase from a low processive to a highly processive enzyme. Processivity is dependent on the presence...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Davidson, John F., Fox, Richard, Harris, Dawn D., Lyons-Abbott, Sally, Loeb, Lawrence A.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2003
Soggetti:	Articles
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169918/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12907710/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg667
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