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Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase

Insertion of the T3 DNA polymerase thioredoxin binding domain (TBD) into the distantly related thermostable Taq DNA polymerase at an analogous position in the thumb domain, converts the Taq DNA polymerase from a low processive to a highly processive enzyme. Processivity is dependent on the presence...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Principais autores: Davidson, John F., Fox, Richard, Harris, Dawn D., Lyons-Abbott, Sally, Loeb, Lawrence A.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2003
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169918/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12907710/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg667
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