Rapid Screening of Topoisomerase Gene Mutations by a Novel Melting Curve Analysis Method for Early Warning of Fluoroquinolone-Resistant Streptococcus pneumoniae Emergence

We developed a real-time PCR assay combined with melting curve analysis for rapidly genotyping quinolone resistance-determining regions (QRDR) of topoisomerase genes in Streptococcus pneumoniae. This assay was not only accurate for the screening of fluoroquinolone (FQ) resistance but also relevant a...

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Bibliographic Details
Main Authors: Fukushima, Kazuko Y., Hirakata, Yoichi, Sugahara, Kazuyuki, Yanagihara, Katsunori, Kondo, Akira, Kohno, Shigeru, Kamihira, Shimeru
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2006
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1698396/
https://ncbi.nlm.nih.gov/pubmed/17021062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01887-06
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