Melting Curve Analysis for Rapid Detection of Topoisomerase Gene Mutations in Haemophilus influenzae

We established a real-time PCR assay with melting curve analysis to rapidly genotype quinolone resistance-determining regions (QRDRs) of gyrase A and topoisomerase IV genes in Haemophilus influenzae. This assay is a useful tool for the detection of fluoroquinolone resistance and for the early detect...

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Bibliografiske detaljer
Main Authors: Nakamura, Shigeki, Yanagihara, Katsunori, Morinaga, Yoshitomo, Izumikawa, Koichi, Seki, Masafumi, Kakeya, Hiroshi, Yamamoto, Yoshihiro, Kamihira, Shimeru, Kohno, Shigeru
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2009
Fag:
Bacteriology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2650933/
https://ncbi.nlm.nih.gov/pubmed/19129413
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01645-08
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