Rapid Identification of Penicillin and Macrolide Resistance Genes and Simultaneous Quantification of Streptococcus pneumoniae in Purulent Sputum Samples by Use of a Novel Real-Time Multiplex PCR Assay

We evaluated a real-time quantitative PCR combined with a multiplex PCR assay for the quantification of Streptococcus pneumoniae and the simultaneous detection of drug-resistant genes by gel-based PCR, using purulent sputum samples. This assay correctly quantified S. pneumoniae and identified their...

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Detalhes bibliográficos
Main Authors: Fukushima, Kazuko Y., Yanagihara, Katsunori, Hirakata, Yoichi, Sugahara, Kazuyuki, Morinaga, Yoshitomo, Kohno, Shigeru, Kamihira, Shimeru
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2446912/
https://ncbi.nlm.nih.gov/pubmed/18463207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00051-08
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