Directed Evolution of Thermus Maltogenic Amylase toward Enhanced Thermal Resistance

The thermostability of maltogenic amylase from Thermus sp. strain IM6501 (ThMA) was improved greatly by random mutagenesis using DNA shuffling. Four rounds of DNA shuffling and subsequent recombination of the mutations produced the highly thermostable mutant enzyme ThMA-DM, which had a total of seve...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Principais autores:	Kim, Young-Wan, Choi, Ji-Hye, Kim, Jung-Wan, Park, Cheonseok, Kim, Jung-Woo, Cha, Hyunju, Lee, Soo-Bok, Oh, Byoung-Ha, Moon, Tae-Wha, Park, Kwan-Hwa
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 2003
Assuntos:	Enzymology and Protein Engineering
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169122/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902281/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4866-4874.2003
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