Directed Evolution of Thermus Maltogenic Amylase toward Enhanced Thermal Resistance

The thermostability of maltogenic amylase from Thermus sp. strain IM6501 (ThMA) was improved greatly by random mutagenesis using DNA shuffling. Four rounds of DNA shuffling and subsequent recombination of the mutations produced the highly thermostable mutant enzyme ThMA-DM, which had a total of seve...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Kim, Young-Wan, Choi, Ji-Hye, Kim, Jung-Wan, Park, Cheonseok, Kim, Jung-Woo, Cha, Hyunju, Lee, Soo-Bok, Oh, Byoung-Ha, Moon, Tae-Wha, Park, Kwan-Hwa
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2003
Soggetti:	Enzymology and Protein Engineering
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169122/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12902281/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.8.4866-4874.2003
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