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Directed Evolution of Thermus Maltogenic Amylase toward Enhanced Thermal Resistance

The thermostability of maltogenic amylase from Thermus sp. strain IM6501 (ThMA) was improved greatly by random mutagenesis using DNA shuffling. Four rounds of DNA shuffling and subsequent recombination of the mutations produced the highly thermostable mutant enzyme ThMA-DM, which had a total of seve...

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Bibliografiske detaljer
Main Authors: Kim, Young-Wan, Choi, Ji-Hye, Kim, Jung-Wan, Park, Cheonseok, Kim, Jung-Woo, Cha, Hyunju, Lee, Soo-Bok, Oh, Byoung-Ha, Moon, Tae-Wha, Park, Kwan-Hwa
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC169122/
https://ncbi.nlm.nih.gov/pubmed/12902281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4866-4874.2003
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