Directed Evolution of Thermus Maltogenic Amylase toward Enhanced Thermal Resistance

The thermostability of maltogenic amylase from Thermus sp. strain IM6501 (ThMA) was improved greatly by random mutagenesis using DNA shuffling. Four rounds of DNA shuffling and subsequent recombination of the mutations produced the highly thermostable mutant enzyme ThMA-DM, which had a total of seve...

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Bibliographische Detailangaben
Hauptverfasser: Kim, Young-Wan, Choi, Ji-Hye, Kim, Jung-Wan, Park, Cheonseok, Kim, Jung-Woo, Cha, Hyunju, Lee, Soo-Bok, Oh, Byoung-Ha, Moon, Tae-Wha, Park, Kwan-Hwa
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2003
Schlagworte:
Enzymology and Protein Engineering
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC169122/
https://ncbi.nlm.nih.gov/pubmed/12902281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.8.4866-4874.2003
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